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A strikingly-angled spindle mediates nuclear migration during colonization of rice cells infected by Magnaporthe oryzae
- Pfeifer, Mariel A., Jones, Kiersun, Khang, Chang Hyun
- Fungal genetics and biology 2019 v.126 pp. 56-60
- Magnaporthe oryzae, blast disease, confocal microscopy, fungal diseases of plants, hyphae, image analysis, interphase, microtubules, mitosis, mitotic spindle apparatus, nuclear membrane, plant pathogenic fungi, rice
- To cause rice blast disease, Magnaporthe oryzae must properly organize microtubules and position nuclei during colonization of host cells. Live cell confocal imaging of fluorescently-tagged microtubules and nuclei of M. oryzae invasive hyphae reveals that microtubules form a cage-like arrangement around nuclei during interphase and that the mitotic spindle forms and mediates nuclear migration while integrity of the nuclear envelope is lost. Our results also unveil a strikingly-angled spindle during nuclear migration through the narrow invasive hyphal peg, suggesting a yet-to-be discovered mechanism of mitotic nuclear migration when invasive hyphae move to adjacent rice cells.