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Economical production of agricultural γ-polyglutamic acid using industrial wastes by Bacillus subtilis

Author:
Zhang, Chao, Wu, Daoji, Ren, Huixue
Source:
Biochemical engineering journal 2019 v.146 pp. 117-123
ISSN:
1369-703X
Subject:
Bacillus subtilis, crystallization, experimental design, glutamic acid, industrial wastes, monosodium glutamate, pollution, pollution control, polyglutamic acid, poultry manure, regression analysis, response surface methodology, soybean cake
Abstract:
Industrial wastes, such as monosodium glutamate waste liquor, chicken manure and soybean cake, were used for agricultural γ-polyglutamic acid (γ-PGA) production by Bacillus subtilis ZC-5. Crude extract of glutamic acid after isoelectric crystallization (CEGA) could be used instead of glutamate for γ-PGA production. The optimal composition of medium was obtained by response surface methodology. Firstly, Plackett-Burman design was undertaken to assess the effects of eight factors. With statistic regression analysis, the significant factors affecting γ-PGA production were determined as follows: chicken manure, soybean cake and CEGA. Then the Box-Behnken design was adopted for further optimization. The optimal addition amounts of factors were determined as: 62.35 g chicken manure, 25.15 g soybean cake and 15.09 g CEGA. Under such condition, the γ-PGA yield was increased to 6.57 ± 0.13%, which was 20.6% higher than the maximum value of the single factor tests. These investigations would lay a foundation for reducing the pollution of industrial wastes, exploring a late-model for agricultural γ-PGA production.
Agid:
6342113