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Biomarkers responses in Manila clam, Ruditapes philippinarum after single and combined exposure to mercury and benzo[a]pyrene

Jiang, Weiwei, Fang, Jianguang, Gao, Yaping, Du, Meirong, Fang, Jinghui, Wang, Xiaoqin, Li, Fengxue, Lin, Fan, Jiang, Zengjie
Comparative biochemistry and physiology 2019 v.220 pp. 1-8
Ruditapes philippinarum, acetylcholinesterase, ammonium nitrogen, aquatic ecosystems, aquatic environment, benzo(a)pyrene, biomarkers, catalase, clams, excretion, glutathione, glutathione transferase, glycogen, hepatopancreas, malondialdehyde, mercury, oxygen consumption, pollutants, superoxide dismutase
Physiological and biochemical responses in bivalves exposed to pollutants have proved a valuable tool to assess the health of organisms in aquatic ecosystems. The single and combined effects of mercury (Hg2+, 2 and 10 μg/L) and benzo[a]pyrene (BaP, 3 μg/L) on physiological and biochemical biomarkers in Manila clam, Ruditapes philippinarum were evaluated. Results showed that significant higher oxygen consumption (OR) and ammonia-N excretion rates (NR) together with significant lower ingestion rates (IR) were observed for the 10 μg/L Hg2+ or 3 μg/L BaP treatments compared to controls (P < 0.05). However, clam NR decreased significantly in response to the binary mixtures of 10 μg/L Hg2+ and 3 μg/L BaP (P < 0.05). Moreover, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferases (GSTs), glutathione (GSH), acetylcholinesterase (AChE) and malondialdehyde (MDA) in the hepatopancreas of clams were induced substantially, whereas glycogen (GLY) contents were suppressed dramatically after Hg2+ and BaP exposure. Additionally, the integrated biomarker response (IBR) values measured showed significant increases in combination treatments and they were much higher than that in the Hg2+ treatment. This study will provide further information on the defense mechanism in the Manila clam after exposure to marine pollutants and may help evaluate the quality of the aquatic environment.