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Decolorization and degradation of methyl orange by Bacillus stratosphericus SCA1007

Akansha, Kriti, Chakraborty, Debashis, Sachan, Shashwati Ghosh
Biocatalysis and agricultural biotechnology 2019 v.18 pp. 101044
Escherichia coli, Fourier transform infrared spectroscopy, Vigna radiata, azo dyes, decolorization, methyl orange, pH, temperature, toxicity testing, ultraviolet-visible spectroscopy, yeast extract
The present study deals with complete decolorization and degradation of sulfonated azo dye (Methyl orange) by Bacillus stratosphericus SCA1007. These classes of dyes are difficult to degrade by conventional treatment methods. To overcome this problem, an environment friendly and efficient method was developed to degrade this recalcitrant dye. Bacillus stratosphericus SCA1007 could carry out complete decolorization of Methyl orange in a wide range up to 150 mg/L of dye concentration, at temperature 35 °C and pH 7 under static condition along with yeast extract in minimal salt media. This is the first study demonstrating complete decolorization of Methyl orange by Bacillus stratosphericus SCA1007 under optimized conditions within 12 h of incubation. The degradation of dye was studied by UV-vis and FTIR. Toxicity studies were performed on Escherichia coli and Vigna radiata to confirm the non-toxic nature of the degraded products.