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Phylogenetic analysis of the key microorganisms shifting in hydrolysis and subsequent fermentation while treating waste activated sludge by thermophilic bacteria

Yang, Chun-Xue, He, Zhang-Wei, Liu, Wen-Zong, Yu, Shao-Peng, Thangavel, Sangeetha, Wang, Ling, Guo, Ze-Chong, Lu, Jin-Yu, Liu, Bao-Ling, Wang, Ai-Jie
Bioresource technology reports 2019 v.6 pp. 168-174
Geobacillus, acidification, activated sludge, amino acids, chemical oxygen demand, community structure, fermentation, genes, hydrolysis, microbial communities, phylogeny, proteins, proteolysis, thermophilic bacteria, volatile fatty acids, waste treatment
Employment of thermophilic bacteria for the pretreatment of waste activated sludge is one of efficient biological processes, which also benefits further sludge digestion. After pretreatment, volatile fatty acids production in the fermentation process was increased to 2521 ± 24 mg COD/L, which was 43% high compared to the control. The shift of microbial community structure from thermophilic pretreatment to hydrolysis might have substantially affected the subsequent WAS acidification. This study has been focused to investigate the links between community structure and functional genes on WAS hydrolysis and acidification after inoculation of Geobacillus sp. G1. The corresponding quantification of genus Geobacillus and proteolytic genes in WAS were explored. Diverse hydrolysis bacteria with a strong ability to degrade proteins and amino acids were detected. Bacterodies were enriched to utilize hydrolysis organics to directly produce organic acids. WAS hydrolysis was efficiently accelerated through pretreatment and volatile fatty acids production was effectively improved.