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The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis

Kiselev, K.V., Grigorchuk, V.P., Ogneva, Z.V., Suprun, A.R., Dubrovina, A.S.
Journal of plant physiology 2019 v.234-235 pp. 133-137
Picea jezoensis, biosynthesis, conifer needles, genes, high performance liquid chromatography, irradiation, p-coumaric acid, stilbenes, transcription (genetics), trihydroxystilbene synthase, ultraviolet radiation
This study examines the effects of p-coumaric (CoA) and caffeic (CaA) acids on stilbene biosynthesis in one-year-old needles of Picea jezoensis (Lindl. et Gord.) Fisch ex Carr. under control conditions and after ultraviolet (UV-C) irradiation. HPLC analysis revealed that while UV-C irradiation slightly affected the total stilbene content, CoA and CaA feeding increased the total content of stilbenes by 1.2–1.3-fold. UV-C treatment combined with CoA-feeding of the P. jezoensis cuttings exerted the most pronounced stimulatory effect on stilbene accumulation leading to the total stilbene content of 9.18 mg/g of dry weight or DW. This increase correlated with the elevated transcription of the stilbene synthase PjSTS1a and PjSTS1b genes. UV-C treatment in combination with CaA feeding of P. jezoensis did not considerably influence stilbene content. These results revealed a positive effect of UV-C radiation and phenolic precursors on the content of stilbenes in spruce needles.