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Structure Elucidation, Conformation, and Configuration of Cytotoxic 6-Heptyl-5,6-dihydro-2H-pyran-2-ones from Hyptis Species and Their Molecular Docking to α-Tubulin

Martínez-Fructuoso, Lucero, Pereda-Miranda, Rogelio, Rosas-Ramírez, Daniel, Fragoso-Serrano, Mabel, Cerda-García-Rojas, Carlos M., da Silva, Aline Soares, Leitão, Gilda Guimarães, Leitão, Suzana Guimarães
Journal of natural products 2019 v.82 no.3 pp. 520-531
Hyptis, Mesosphaerum pectinatum, altitude, computer simulation, countercurrent chromatography, cytotoxicity, high performance liquid chromatography, indigenous species, medicinal properties, nuclear magnetic resonance spectroscopy, traditional medicine, tubulin
Cytotoxic 6-heptyl-5,6-dihydro-2H-pyran-2-ones are chemical markers of Hyptis (Lamiaceae) and are responsible for some of the therapeutic properties of species with relevance to traditional medicine. The present investigation describes the isolation of known pectinolides A–C (1–3), in addition to the new pectinolides I–M (4–8), from two Mexican collections of H. pectinata by HPLC. The novel biosynthetically related monticolides A (9) and B (10) were also isolated by high-speed countercurrent chromatography from H. monticola, an endemic species of the Brazilian southeastern high-altitude regions. A combination of chemical correlations, chiroptical measurements, and Mosher ester NMR analysis was used to confirm their absolute configuration. The utility of DFT-NMR chemical shifts and JH–H calculations was assessed for epimer differentiation. Molecular docking studies indicated that 6-heptyl-5,6-dihydro-2H-pyran-2-ones have a high affinity for the pironetin-binding site of α-tubulin, which may be a possible mechanism contributing to the cytotoxic potential of these small and flexible molecules.