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Titanium discs coated with 3,4-dihydroxy-l-phenylalanine promote osteogenic differentiation of human bone mesenchymal stem cells in vitro
- Ma, Ting, Ge, Xi-Yuan, Hao, Ke-Yi, Jiang, Xi, Zheng, Yan, Lin, Ye, Zhang, Yu
- RSC advances 2019 v.9 no.16 pp. 9117-9125
- 3,4-dihydroxyphenylalanine oxidative deaminase, alkaline phosphatase, bone formation, cell adhesion, cell proliferation, coatings, enzyme activity, focal adhesions, gene expression regulation, genes, humans, mesenchymal stromal cells, microarray technology, mineralization, tissue engineering, titanium
- The bioinspired material 3,4-dihydroxy-l-phenylalanine (DOPA) is commonly used as a basic layer in surface modification for osteogenesis; however, its effects on bone remodeling and the underlying mechanisms remain unclear. Here, we investigated the effect of DOPA-coated surfaces on human bone marrow-derived mesenchymal stem cells in vitro. Cells cultured on DOPA-modified titanium discs exhibited enhanced cellular adhesion and spreading compared with cells on non-treated surfaces. Moreover, DOPA-coating promoted greater cell proliferation and osteogenic differentiation, as determined using cell counting kit-8 (CCK-8) assay, alkaline phosphatase activity test and quantitative mineralization measurements. Furthermore, microarray analysis revealed that genes participating in focal adhesion were upregulated on DOPA-coated surfaces. Our results indicate that the application of a simple DOPA coating can promote osteogenic differentiation of osteoprogenitor cells, improving new bone formation and bone remodeling around implantable devices in tissue engineering.