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Titanium discs coated with 3,4-dihydroxy-l-phenylalanine promote osteogenic differentiation of human bone mesenchymal stem cells in vitro

Author:
Ma, Ting, Ge, Xi-Yuan, Hao, Ke-Yi, Jiang, Xi, Zheng, Yan, Lin, Ye, Zhang, Yu
Source:
RSC advances 2019 v.9 no.16 pp. 9117-9125
ISSN:
2046-2069
Subject:
3,4-dihydroxyphenylalanine oxidative deaminase, alkaline phosphatase, bone formation, cell adhesion, cell proliferation, coatings, enzyme activity, focal adhesions, gene expression regulation, genes, humans, mesenchymal stromal cells, microarray technology, mineralization, tissue engineering, titanium
Abstract:
The bioinspired material 3,4-dihydroxy-l-phenylalanine (DOPA) is commonly used as a basic layer in surface modification for osteogenesis; however, its effects on bone remodeling and the underlying mechanisms remain unclear. Here, we investigated the effect of DOPA-coated surfaces on human bone marrow-derived mesenchymal stem cells in vitro. Cells cultured on DOPA-modified titanium discs exhibited enhanced cellular adhesion and spreading compared with cells on non-treated surfaces. Moreover, DOPA-coating promoted greater cell proliferation and osteogenic differentiation, as determined using cell counting kit-8 (CCK-8) assay, alkaline phosphatase activity test and quantitative mineralization measurements. Furthermore, microarray analysis revealed that genes participating in focal adhesion were upregulated on DOPA-coated surfaces. Our results indicate that the application of a simple DOPA coating can promote osteogenic differentiation of osteoprogenitor cells, improving new bone formation and bone remodeling around implantable devices in tissue engineering.
Agid:
6345039