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Simultaneous determination of 169 veterinary drugs in chicken eggs with EMR-Lipid clean-up using ultra-high performance liquid chromatography tandem mass spectrometry

Author:
Luo, Ping, Liu, Xiaohong, Kong, Fang, Chen, Liangkai, Wang, Qiang, Li, Wanyi, Wen, Sheng, Tang, Lin, Li, Yonggang
Source:
Analytical methods 2019 v.11 no.12 pp. 1657-1662
ISSN:
1759-9679
Subject:
acetonitrile, chicken eggs, detection limit, drug residues, drying, formic acid, high performance liquid chromatography, multiresidue analysis, pouches, solid phase extraction, standard deviation, tandem mass spectrometry, ultra-performance liquid chromatography, veterinary drugs, China
Abstract:
A multiresidue method for the simultaneous determination of 169 veterinary drugs in chicken egg was developed and validated according to the requirements of the China Conformity Assessment GB/T 27417-2017. The method was developed by extraction using acetonitrile with 5% formic acid and then using enhanced matrix removal-lipid in dispersive solid phase extraction and drying pouches for further clean-up with ultra-high performance liquid chromatography tandem mass spectrometry of an electrospray–ionization interface in positive ion mode. The limits of detection and quantification of the analytes were 0.01–3.81 μg kg⁻¹ and 0.03–12.57 μg kg⁻¹, respectively. For the analytes, the recoveries were 57–124%, and the relative standard deviations of all analytes ranged from 2% to 31%. Linearity was evaluated by performing matrix-matched calibration at 0.1, 0.2, 0.5, 1, 5, 10, 20, and 40 μg kg⁻¹ levels. The evaluated method enabled the rapid, reliable, and robust quantification and identification of 169 veterinary drug residues and was successfully applied in real samples.
Agid:
6345064