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Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States
- Allard, Sarah M., Solaiman, Sultana, Callahan, Mary Theresa, Bui, Anthony, Craddock, Hillary, Haymaker, Joseph, Foust, Derek, Duncan, Rico, Smyth, Eoghan, Mongodin, Emmanuel F., Hashem, Fawzy, May, Eric, Micallef, Shirley A., Sapkota, Amy R.
- Environmental research 2019 v.172 pp. 296-300
- bacterial communities, die-off, microorganisms, ribosomal RNA, sequence analysis, sodium, thiosulfates, wastewater treatment, United States
- A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching.