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Transformation of L-Tyrosine to L-Dopa by a Novel Fungus, Acremonium rutilum, Under Submerged Fermentation

Krishnaveni, R., Rathod, Vandana, Thakur, M. S., Neelgund, Y. F.
Current microbiology 2009 v.58 no.2 pp. 122-128
Acremonium, L-dopa, Parkinson disease, agar, bananas, catechol oxidase, fungi, inoculum, pH, screening, submerged fermentation, temperature
The present study deals with the transformation of L-tyrosine to L-dopa by Acremonium rutilum, a fungal tyrosinase producer, isolated from decomposed banana stud. This appears to be the first report on A. rutilum as a polyphenoloxidase producer with both cresolase and catecholase activity. Enriched Czapek-Dox agar was used for plate assay screening. Enriched potato dextrose broth was used for optimization studies, which induced high levels of L-dopa under submerged fermentation. A. rutilum gave the maximum L-dopa production (0.89 mg/ml) and tyrosinase activity (1095 U/mg) under the optimized parameters, that is, a temperature of 25°C, pH 5.5, an inoculum size of 2.5 ml, and an incubation time of 72-120 h, with L-tyrosine (5 mg/ml) as substrate. Five resolved bands, with Rf values of 0.73, 0.60, 0.54, 0.37, and 0.26, were observed, which confirmed the presence of L-dopa. This study involves the elevated profile of L-dopa production. Such study is needed, as L-dopa has the ability to control Parkinson's disease.