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RNA-binding protein RBM6 as a tumor suppressor gene represses the growth and progression in laryngocarcinoma

Wang, Qiwei, Wang, Fang, Zhong, Waisheng, Ling, Hang, Wang, Jixuan, Cui, Jie, Xie, Tao, Wen, Senli, Chen, Jie
Gene 2019 v.697 pp. 26-34
RNA-binding proteins, apoptosis, caspase-3, cell viability, gene expression, human cell lines, humans, messenger RNA, mitogen-activated protein kinase, neoplasms, patients, protein synthesis, tumor suppressor genes
Aberrant expression of RBM6 has been implicated in the development of human malignancies. However, the bio-function of RBM6 in laryngocarcinoma is still almost blank. Here we identified that RBM6 was downregulated in laryngocarcinoma tissues, as well as laryngocarcinoma cell lines. Notably, the expression level of RBM6 was lower in laryngocarcinoma patients at stage3/4 than that in laryngocarcinoma patients at stage1/2. Upregulation of RBM6 suppressed the proliferation of TU212 and Hep-2 cells, as shown by decreased cell viability and Ki67 level. In parallel, overexpression of RBM6 inhibited invasion and promoted apoptosis of TU212 and Hep-2 cells, as evidenced by downregulation of MMP-2 and MMP-9 protein expression and upregulation of cleaved caspase-3 protein expression. In vivo, RBM6 overexpression repressed the laryngocarcinoma tumor growth. EGFR mRNA level was higher in the laryngocarcinoma tissues than that in the adjacent normal tissues. Moreover, upregulation of RBM6 reduced the expression of EGFR, ERK and p-ERK in vitro and in vivo. Our data suggest that RBM6 as a tumor suppressor represses the growth and progression in laryngocarcinoma.