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Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli

Author:
Kim, Susie, Moon, Doo-Bum, Lee, Chung-Hwan, Nam, Soo-Wan, Kim, Pil
Source:
Current microbiology 2009 v.58 no.2 pp. 159-163
ISSN:
0343-8651
Subject:
Escherichia coli, NAD (coenzyme), NAD(P)H dehydrogenase (quinone), NADP (coenzyme), cell growth, dyes, glucose
Abstract:
To better understand how the reducing power of either NADH or NADPH affects cell growth, Escherichia coli strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h⁻¹, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.
Agid:
634643