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Identification and characterisation of Staphylococcus aureus on low cost screen printed carbon electrodes using impedance spectroscopy

Author:
Ward, A.C., Hannah, A.J., Kendrick, S.L., Tucker, N.P., MacGregor, G., Connolly, P.
Source:
Biosensors & bioelectronics 2018 v.110 pp. 65-70
ISSN:
0956-5663
Subject:
Staphylococcus aureus, adsorption, bacteria, biosensors, carbon electrodes, dielectric spectroscopy, electrochemistry, metabolism, morbidity, mortality, sodium chloride
Abstract:
Staphylococcus aureus infections are a cause of significant morbidity and mortality, in addition to representing a considerable economic burden. The aim of this study was to explore a low cost screen printed electrode as a sensor for the detection of S. aureus using impedance spectroscopy. S. aureus was incubated in chambers containing the electrodes and the results analysed using a novel normalisation approach. These results show that it is possible to detect the presence of S. aureus in LB media after 30 min incubation of a 1% growth culture, in addition to being able to see immediate cell concentration dependant changes in 0.9% NaCl. These observations imply that a number of electrochemical mechanisms cause a change in the impedance as a result of the presence of S. aureus, including adsorption to the electrode surface and the metabolism of the bacteria during growth. The study suggests that this detection approach would be useful in a number of clinical scenarios where S. aureus leads to difficult to treat infections.
Agid:
6346749