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Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells
- Yoon, Jong Hyun, Roh, Eun Youn, Shin, Sue, Jung, Nam Hee, Song, Eun Young, Lee, Dong Soon, Han, Kyou Sup, Kim, Joung Sung, Kim, Byoung Jae, Jeon, Hye Won, Yoon, Kang Sup
- Biotechnology letters 2009 v.31 no.3 pp. 329-335
- adults, humans, stem cells, ultrasonics, umbilical cord
- The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10⁵ cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.