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Nitric oxide treatment maintains postharvest quality of table grapes by mitigation of oxidative damage

Author:
Zhang, Zheng, Xu, Jing, Chen, Yan, Wei, Jia, Wu, Bin
Source:
Postharvest biology and technology 2019 v.152 pp. 9-18
ISSN:
0925-5214
Subject:
Vitis vinifera, ascorbic acid, catalase, enzyme activity, fruit peels, fruit pulp, fruit quality, gene expression, genes, glutathione-disulfide reductase, hydrogen peroxide, lipid peroxidation, metabolism, nitric oxide, peroxidase, storage quality, storage temperature, superoxide anion, superoxide dismutase, table grapes
Abstract:
The senescence of table grape is closely related to reactive oxygen species (ROS) damage. In this study, the mechanism of NO on mitigating the oxidative damage of table grape (Vitis vinifera L.) during storage at 0 °C for 60 days was explored by quality, antioxidant enzyme activity and related gene expression. Our result showed that fruit quality was maintained by NO with the concentration of 300 μL L−1. NO treatment alleviated ROS accumulation and membrane lipid peroxidation by activated superoxide dismutase (SOD), ascorbic acid peroxidase (APX), catalase (CAT), peroxidase (POD) and glutathione reductase (GR) activities in peel and pulp. The accumulation of superoxide anion (O2−) and hydrogen peroxide (H2O2), as well as the activities of some antioxidant enzymes were different in peel and pulp. The results of q-PCR showed that NO treatment induced VvSOD, VvCAT, VvPOD2 and VvGR expression and VvSOD and VvCAT were the main genes expressed in peel and pulp. Therefore, NO regulated the ROS metabolism in peel and pulp was a possible mechanism for mitigating the oxidative damage of table grape.
Agid:
6348160