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Optimization of encapsulation of a microbial laccase enzymatic extract using selected matrices

Gill, Jagpreet, Orsat, Valerie, Kermasha, Selim
Process biochemistry 2018 v.65 pp. 55-61
Coriolus hirsutus, alginates, catalytic activity, encapsulation, enzyme activity, laccase, silica, storage quality, storage time
The encapsulation of the laccase extract, obtained from Coriolus hirsutus, was investigated using selected matrices, including alginate, alginate-silica and silica sol-gel. Two methods, M1 and M2, were carried out for the hybridization of alginate with silica. The experimental findings indicated that the incorporation of silica into alginate, using M2, resulted by a higher (70%) encapsulation efficiency (EE) for the laccase extract as compared to that for the alginate (59%) alone. Furthermore, the encapsulation of the laccase extract in sol-gel resulted in an enhancement of its catalytic activity, with a concomitant increase (90%) in the EE. The alginate and sol-gel matrices also enhanced the laccase catalytic efficiency as compared to that for the free one, with a kcat of 89.9, 63.7 and 56.9min−1, respectively. The protein release profiles indicated that the laccase was more effectively confined in the sol-gel and alginate-silica M2 as compared to that for the alginate and alginate-silica M1. In addition, the laccase extract in sol-gel exhibited an enhanced re-usability, with a relative residual enzyme activity of 82.7%, after 5 continuous reaction cycles. The alginate, alginate-silica M2 and sol-gel matrices demonstrated complete storage stability at 4°C, without any loss in laccase activity after 6 weeks of storage.