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Kinetics of type I methanotrophs mixed culture enriched from waste activated sludge
- AlSayed, Ahmed, Fergala, Ahmed, Khattab, Saif, Eldyasti, Ahmed
- Biochemical engineering journal 2018 v.132 pp. 60-67
- Methylobacter, Methylococcus, Methylomicrobium, Methylomonas, Methylosarcina, Methylosphaera, activated sludge, biodegradation, biotechnology, growth models, methane, methanotrophs, microbial growth, mixed culture, products and commodities, specific growth rate, wastewater treatment
- Methanotrophs are unique microorganisms that can upgrade the methane produced within wastewater treatment plants into diverse prominent commodities. The determination of the methanotrophic enrichments growth kinetics is a pivotal milestone to develop any methane based biotechnology. In this study, diversified batch incubations were performed to experimentally obtain, for the first time, the microbial growth kinetics for type I methanotrophs enriched from waste activated sludge. The key genus in the enriched culture includes Methylococcus, Methylobacter, Methaylocaldum, Methylomicrobium, Methylomonas, Methylosarcina, Methylosphaera. The attained maximum specific growth rate (μmax) (0.358 h−1) and maximum specific methane biodegradation rate (qmax) (g-CH4,Total g-DCW−1 h−1) were the highest reported in mixed cultures. Furthermore, the low substrate threshold concentration (Smin) (0.033 mg-CH4,aq./hr) observed shows that the microbial activity can be maintained at a very low methane aqueous concentrations. The obtained kinetic parameters confirm the prominence of utilizing type I methanotrophs mixed cultures in resources recovery processes from waste streams.