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Production and characterization of ectoine using a moderately halophilic strain Halomonas salina BCRC17875

Author:
Chen, Wei-Chuan, Hsu, Ching-Cha, Lan, John Chi-Wei, Chang, Yu-Kaung, Wang, Li-Fen, Wei, Yu-Hong
Source:
Journal of bioscience and bioengineering 2018 v.125 no.5 pp. 578-584
ISSN:
1389-1723
Subject:
Halomonas salina, agitation, ammonium sulfate, carbon, carbon nitrogen ratio, chemical structure, commercialization, fermentation, mass spectrometry, nitrogen, nitrogen content, nuclear magnetic resonance spectroscopy, sodium chloride, yeast extract
Abstract:
This study attempted to utilize Halomonas salina BCRC17875 to produce ectoine by optimizing the agitation speed and medium composition. In addition, the chemical structure of ectoine produced by H. salina BCRC17875 was determined. The results indicate that ectoine production reached 3.65 g/L at 38 h of cultivation when the agitation rate and NaCl concentration were fixed at 200 rpm and 2.0 M, respectively. It reached 9.20 g/L at 44 h of cultivation when the major medium components were yeast extract (56 g/L), glutamate (74.40 g/L), and ammonium sulfate (14 g/L). After the nitrogen concentration had been evaluated, evaluation of the nitrogen concentration revealed that the ectoine production reached 11.80 g/L at 44 h of cultivation when 56 g/L of yeast extract and 28 g/L of ammonium sulfate were used. Ectoine production reached 13.96 g/L at 44 h of cultivation when the carbon/nitrogen ratio was fixed at 3/1 using 84 g/L of yeast extract and 28 g/L of ammonium sulfate. Furthermore, the identification of ectoine were identified and characterized by fast atom bombardment mass spectrometry (FAB-MS) and 1H NMR. The results demonstrated a fermentation strategy was successful in increasing ectoine production, and that the fermentation medium of ectoine had commercialization potential.
Agid:
6348566