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CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome

Author:
Hao, Li, Ruiying, Qin, Xiaoshuang, Liu, Shengxiang, Liao, Rongfang, Xu, Jianbo, Yang, Pengcheng, Wei
Source:
Rice science 2019 v.26 no.2 pp. 125-128
ISSN:
1672-6308
Subject:
DNA shuffling, RNA, adenine, genes, genomics, point mutation, rice, single nucleotide polymorphism
Abstract:
Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.
Agid:
6348703