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Report and genetic identification of Amyloodinium ocellatum in a sea bass (Dicentrarchus labrax) broodstock in Portugal

Author:
Marques, Cátia L., Medeiros, Ana, Moreira, Márcio, Quental-Ferreira, Hugo, Mendes, Ana C., Pousão-Ferreira, Pedro, Soares, Florbela
Source:
Aquaculture reports 2019 v.14 pp. 100191
ISSN:
2352-5134
Subject:
Amyloodinium ocellatum, Dicentrarchus labrax, amyloodiniosis, bass, breeding stock, epithelium, genes, gills, histology, hyperplasia, hypertrophy, microscopy, parasites, protists, ribosomal RNA, sequence analysis, species identification, Portugal
Abstract:
In this paper we report a case of amyloodiniosis in a sea bass (Dicentrarchus labrax) broodstock in Portugal. Microscopic examination of gill filaments showed the presence of trophonts while histological observation revealed gills epithelial hyperplasia, hypertrophy and lamellar fusion of secondary lamellae. The amplification and sequencing of the small subunit ribosomal RNA gene allowed the identification of the parasite as Amyloodinium ocellatum. It was also possible to amplify a partial sequence of ribosomal RNA from a Colpodellid, a predator of protists.
Agid:
6348815