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Extensive Post-Transcriptional Regulation Revealed by Transcriptomic and Proteomic Integrative Analysis in Cassava under Drought

Ding, Zehong, Fu, Lili, Tie, Weiwei, Yan, Yan, Wu, Chunlai, Hu, Wei, Zhang, Jiaming
Journal of agricultural and food chemistry 2019 v.67 no.12 pp. 3521-3534
biosynthesis, cassava, drought, food crops, genes, heat shock proteins, leaves, messenger RNA, protein content, protein synthesis, proteomics, roots, sequence analysis, transcriptomics, water stress
Cassava is a major tropical/subtropical food crop and its yield is greatly restrained by drought; however, the mechanism underlying the drought stress remains largely unknown. In this study, totally 1242 and 715 differentially expressed genes (DEGs), together with 237 and 307 differentially expressed proteins (DEPs), were respectively identified in cassava leaves and roots through RNA-seq and iTRAQ techniques. The majority of DEGs and DEPs were exclusively regulated at the mRNA and protein level, respectively, whereas only a few were commonly regulated, indicating the major involvement of post-transcriptional regulation under drought. Subsequently, the functions of these specifically or commonly regulated DEGs and DEPs were analyzed, and the post-transcriptional regulation of genes involved in heat shock protein, secondary metabolism biosynthesis, and hormone biosynthesis was extensively discussed. This is the first report on an integration of transcriptomic and proteomic analysis in cassava, and it provides new insights into the post-transcriptional regulation of cassava drought stress.