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Licochalcone B Extracted from Glycyrrhiza uralensis Fisch Induces Apoptotic Effects in Human Hepatoma Cell HepG2

Wang, Jun, Liao, Ai-Mei, Thakur, Kiran, Zhang, Jian-Guo, Huang, Ji-Hong, Wei, Zhao-Jun
Journal of agricultural and food chemistry 2019 v.67 no.12 pp. 3341-3353
Glycyrrhiza uralensis, apoptosis, caspase-8, caspase-9, cell cycle checkpoints, enzyme inhibition, food composition, functional foods, hepatoma, high performance liquid chromatography, humans, inhibitory concentration 50, messenger RNA, mitochondria, nuclear magnetic resonance spectroscopy, protein content, proteins, tandem mass spectrometry
The present study explored the molecular mechanism by which licochalcone B induces the cell cycle arrest and apoptosis in human hepatoma cell HepG2. Initial extraction and identification were performed by HPLC, UPLC-TOF-MS/MS, and NMR analysis, respectively. Licochalcone B inhibited the HepG2 growth with IC₅₀ (110.15 μM) after 24 h, caused morphological distortion, and seized the cell cycle in the G2/M phase (cell arrest in G2/M:43.1 ± 2.2% for 120 μM versus 23.7 ± 1.2% for control), as well as induced apoptosis and intracellular ROS generation. Furthermore, exposure to licochalcone B markedly affected the cell cycle (up/down regulation) at mRNA and protein levels. Apoptosis was induced through the activation of receptor-mediated and mitochondrial pathways. The inhibition of Caspase 8 and Caspase 9 proteins abolished the licochalcone B induced apoptosis. The present work suggested that licochalcone B may further be identified as a potent functional food component with specific health benefits.