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Rapid Identification of Biofilms Using a Robust Multichannel Polymer Sensor Array
- Ngernpimai, Sawinee, Geng, Yingying, Makabenta, Jessa Marie, Landis, Ryan F., Keshri, Puspam, Gupta, Akash, Li, Cheng-Hsuan, Chompoosor, Apiwat, Rotello, Vincent M.
- ACS applied materials & interfaces 2019 v.11 no.12 pp. 11202-11208
- bacteria, biofilm, coculture, fluorescence, fluorescent dyes, mammals, models, polymers
- Infections caused by bacterial biofilms are challenging to diagnose because of the complexity of both the bacteria and the heterogeneous biofilm matrix. We report here a robust polymer-based sensor array that uses selective interactions between polymer sensor elements and the biofilm matrix to identify bacteria species. In this array, an appropriate choice of fluorophore enabled excimer formation and interpolymer FRET, generating six output channels from three polymers. Selective multivalent interactions of these polymers with the biofilm matrices caused differential changes in fluorescent patterns, providing a species-based signature of the biofilm. The real-world potential of the platform was further validated through identification of mixed-species bacterial biofilms and discrimination of biofilms in a mammalian cell-biofilm co-culture wound model.