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Tridimensional alginate disks of tunable topologies for mammalian cell encapsulation

Author:
Suarez-Arnedo, Alejandra, Narváez, Diana M., Sarmiento, Paula, Bocanegra, Laura, Salcedo, Felipe, Muñoz-Camargo, Carolina, Groot, Helena, Cruz, Juan C.
Source:
Analytical biochemistry 2019 v.574 pp. 31-33
ISSN:
0003-2697
Subject:
alginates, calcium, encapsulation, gelation, hydrogels, mammals, mechanical properties, structure-activity relationships, topology
Abstract:
We have developed a protocol to produce three-dimensional matrices based on alginate hydrogels for mammalian cell encapsulation. Based on the gelation properties of this polysaccharide, we implemented a calcium ion-based diffusion method where the designed hydrogels can be obtained with well-defined mechanical properties and replicable 3D topologies. The developed protocol can be extended to different types of alginates and an ample range of concentrations. This makes it very attractive for various biomedical applications where strict control over structure-function relationships is desirable.
Agid:
6350104