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Long non-coding RNA LOC730100 enhances proliferation and invasion of glioma cells through competitively sponging miR-760 from FOXA1 mRNA

Author:
Li, Qun, Lu, Jianglong, Xia, Jia, Wen, Min, Wang, Chengde
Source:
Biochemical and biophysical research communications 2019 v.512 no.3 pp. 558-563
ISSN:
0006-291X
Subject:
apoptosis, cell lines, central nervous system, cytoplasm, glioma, loss-of-function mutation, messenger RNA, non-coding RNA, patients, prognosis, tissues
Abstract:
Glioma is the most malignant cancer in central nervous system. And researchers have indicated that long noncoding RNAs (lncRNAs) are closely related with glioma progression. Nevertheless, LOC730100 function in glioma is ill studied. In the current research, we showed that LOC730100 expression was increased in glioma tissues and cell lines. Furthermore, LOC730100 upregulation is linked to a poor prognosis in glioma patients. Loss-of-function assays showed that LOC730100 knockdown inhibited proliferation, migration and invasion of glioma cells, but increasing apoptosis. Notably, we found that LOC730100 was mainly localized in the cytoplasm of glioma cells. We demonstrated that LOC730100 was a competing endogenous RNA (ceRNA) for miR-760 and promoted the expression of FOXA1. We proved that miR-760 suppresses glioma progression and FOXA1 overexpression reversed it. In conclusion, our findings revealed that LOC730100 promoted glioma progression through regulating miR-760/FOXA1 axis.
Agid:
6350146