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EID1 plays a crucial role in proliferation of neural stem cell

Author:
Fu, Xiaojun, Ding, Boyun, Wang, Chongwu, Chen, Chen, Wang, Ji, Fei, Xiaowei, Xu, Ruxiang
Source:
Biochemical and biophysical research communications 2019 v.512 no.4 pp. 763-769
ISSN:
0006-291X
Subject:
Western blotting, acetyltransferases, bioinformatics, brain, cell proliferation, enzyme activity, memory, mice, neural stem cells, phosphatidylinositol 3-kinase
Abstract:
EP300-interacting inhibitor of differentiation 1 (Eid1) regulates differentiation, transcription and acetyltransferase activity. But the main function of Eid1 in the brain is still unclear. To better understand this issue, we generated Eid1-knockout (Eid1-KO) mice. We found poorer learning and memory ability, and smaller volume of neonatal telencephalon in Eid1-KO group than wild-type (WT). Bioinformatics implied that Eid1 may directly regulate cell proliferation. We then isolated neural stem cells (NSCs) and discovered a slower proliferation rate in Eid1-KO NSCs. Moreover, based on bioinformatics results, we investigated the expression of phosphatidylinositol 3-kinase (PI3K)/AKT/GSK3β pathway by Western blotting assay, which showed attenuated in Eid1-KO group. Our data proved the first comprehensive report of Eid1 regulating NSCs proliferation via PI3K/AKT/GSK3β pathway, and provide a foundation for the role of EID1 in the brain.
Agid:
6350160