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EID1 plays a crucial role in proliferation of neural stem cell

Fu, Xiaojun, Ding, Boyun, Wang, Chongwu, Chen, Chen, Wang, Ji, Fei, Xiaowei, Xu, Ruxiang
Biochemical and biophysical research communications 2019 v.512 no.4 pp. 763-769
Western blotting, acetyltransferases, bioinformatics, brain, cell proliferation, enzyme activity, memory, mice, neural stem cells, phosphatidylinositol 3-kinase
EP300-interacting inhibitor of differentiation 1 (Eid1) regulates differentiation, transcription and acetyltransferase activity. But the main function of Eid1 in the brain is still unclear. To better understand this issue, we generated Eid1-knockout (Eid1-KO) mice. We found poorer learning and memory ability, and smaller volume of neonatal telencephalon in Eid1-KO group than wild-type (WT). Bioinformatics implied that Eid1 may directly regulate cell proliferation. We then isolated neural stem cells (NSCs) and discovered a slower proliferation rate in Eid1-KO NSCs. Moreover, based on bioinformatics results, we investigated the expression of phosphatidylinositol 3-kinase (PI3K)/AKT/GSK3β pathway by Western blotting assay, which showed attenuated in Eid1-KO group. Our data proved the first comprehensive report of Eid1 regulating NSCs proliferation via PI3K/AKT/GSK3β pathway, and provide a foundation for the role of EID1 in the brain.