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Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii

Kang, Min-Cheol, Lee, HyoGeun, Choi, Hee-Don, Jeon, You-Jin
International journal of biological macromolecules 2019 v.132 pp. 142-149
Danio rerio, Sargassum thunbergii, antioxidant activity, cell lines, chromatography, cosmetics, free radical scavengers, functional foods, hydrogen peroxide, ion exchange, oxidative stress, polysaccharides, protective effect, proteinases
The protective effect of sulfated polysaccharides (PS-4) isolated from Sargassum thunbergii against oxidative stress in cells and zebrafish was investigated. To begin, a variety of enzymatic extraction methods were used, including five carbohydrases and four proteases. Among the enzymatic extracts, the S. thunbergii Celluclast-assisted extract (STC) demonstrated the strongest scavenging activities against hydrogen peroxide (H2O2). Five polysaccharide fraction (PS-1 to 5) were isolated from STC and purified using ion exchange column chromatography. The antioxidant activity of these fractions was then assessed. PS-4 demonstrated the most promising free radical scavenging and antioxidant activity. PS-4 had a higher content of sulfated polysaccharide than STC. Thus, increasing the proportion of sulfated polysaccharides may be attributed to protection against H2O2-mediated cell damage and oxidative stress in cell lines and in zebrafish. These results suggest that PS-4 could be a potent antioxidant agent in functional foods and cosmetics.