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Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii

Author:
Kang, Min-Cheol, Lee, HyoGeun, Choi, Hee-Don, Jeon, You-Jin
Source:
International journal of biological macromolecules 2019 v.132 pp. 142-149
ISSN:
0141-8130
Subject:
Danio rerio, Sargassum thunbergii, antioxidant activity, cell lines, chromatography, cosmetics, free radical scavengers, functional foods, hydrogen peroxide, ion exchange, oxidative stress, polysaccharides, protective effect, proteinases
Abstract:
The protective effect of sulfated polysaccharides (PS-4) isolated from Sargassum thunbergii against oxidative stress in cells and zebrafish was investigated. To begin, a variety of enzymatic extraction methods were used, including five carbohydrases and four proteases. Among the enzymatic extracts, the S. thunbergii Celluclast-assisted extract (STC) demonstrated the strongest scavenging activities against hydrogen peroxide (H2O2). Five polysaccharide fraction (PS-1 to 5) were isolated from STC and purified using ion exchange column chromatography. The antioxidant activity of these fractions was then assessed. PS-4 demonstrated the most promising free radical scavenging and antioxidant activity. PS-4 had a higher content of sulfated polysaccharide than STC. Thus, increasing the proportion of sulfated polysaccharides may be attributed to protection against H2O2-mediated cell damage and oxidative stress in cell lines and in zebrafish. These results suggest that PS-4 could be a potent antioxidant agent in functional foods and cosmetics.
Agid:
6350842