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Rumen-derived lipopolysaccharide induced ruminal epithelium barrier damage in goats fed a high-concentrate diet

Author:
Zhang, Kai, Meng, Meijuan, Gao, Lipeng, Tu, Yuanlu, Bai, Yunfeng
Source:
Microbial pathogenesis 2019 v.131 pp. 81-86
ISSN:
0882-4010
Subject:
Boer (goat breed), animal feeding, diet, gene expression, lipopolysaccharides, messenger RNA, mitogen-activated protein kinase, occludins, pH, protein content, protein synthesis, rumen epithelium, rumen fluids, signal transduction, staining, tight junctions
Abstract:
This study aimed to investigate the mechanism of lipopolysaccharide (LPS) released in the rumen on epithelium barrier function of goats fed a HC diet. Twelve Boer goats were randomly divided into two groups: low-concentrate(LC) diet and high-concentrate(HC) diet treatment. We found that the pH of rumen fluid in the HC group was lower than in the LC group (P < 0.05). The mRNA and protein expression levels of p38 mitogen-activated protein kinase (MAPK), extracellular regulated protein kinases (ERK), and c-Jun N-terminal kinase (JNK) in the rumen epithelium were lower in the LC group than the HC group (P < 0.05). Gene expression and protein levels of the tight junction proteins claudin-1, claudin-4, occludin, and Zona occludin-1 were all greater in the LC group than the HC group (P < 0.05). Staining of claudin-1, occludin and ZO-1 was became irregular. In conclusion, high concentrate diet feeding can impair rumen epithelium function and decrease tight junction protein expression through MAPK signaling pathway.
Agid:
6351793