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Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis

Guo, Ling, Jiang, Xiao, Tian, Hao-Yuan, Yao, Shang-Jin, Li, Bo-Ya, Zhang, Rong-Jie, Zhang, Shu-Sheng, Sun, Xin
Yàowù shípǐn fēnxī 2019 v.27 no.2 pp. 518-525
DNA, benzo(a)pyrene, blood, blood sampling, carcinogenesis, carcinogenicity, congenital abnormalities, humans, liquid chromatography, metabolism, metabolites, models, mutagenicity, polycyclic aromatic hydrocarbons, tandem mass spectrometry, teratogenicity, umbilical cord
Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups.