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Molecular characterization of Cyprinid herpesvirus 3 encoded viral interleukin10

Author:
Ouyang, Ping, Yang, Ruixue, Yin, Lizi, Geng, Yi, Lai, Weiming, Huang, Xiaoli, Chen, Defang, Fang, Jing, Chen, Zhengli, Tang, Li, He, Min, Huang, Chao, Liu, Wentao, Wang, Kaiyu
Source:
Fish & shellfish immunology 2019 v.89 pp. 149-157
ISSN:
1050-4648
Subject:
Cyprinid herpesvirus 3, Cyprinus carpio, amino acid sequences, bioactive properties, bioinformatics, genes, immune evasion, interleukin-10, koi, quantitative polymerase chain reaction, viruses
Abstract:
Cyprinid herpesvirus 3 (CyHV-3), a virus that encodes an interleukin10 (IL-10) homologue, causes severe economic losses to the common carp and koi culture industry. The present study was devoted to this IL-10 homologue. Recombinant viral IL-10 (vIL-10) protein encoded by CyHV-3 ORF134 gene using prokaryotic expression system was obtained successfully. Bioinformatics analysis revealed that the amino acid sequence of CyHV-3 vIL-10 has low homology with other host IL-10 or viruses encoded IL-10s. However, their tertiary structure is quite similar, suggesting conservative biological functions between IL-10s and vIL-10s. The biological activity of CyHV-3 vIL-10 was detected by using CCK-8 kit and real time quantitative PCR. The results showed that CyHV-3 vIL-10 down regulate epithelioma papulosum cyprini (EPC) cellular activity at 72 h. Moreover, CyHV-3 vIL-10 inhibits the LPS-induced expression of proinflammatory genes, similar to common carp IL-10. Altogether, the results of this study demonstrate that a clear biological activity of CyHV-3 vIL-10 on its host cells and indicates CyHV-3 vIL-10 may play an important role in viral immune evasion.
Agid:
6352292