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Molecular characterization of Cyprinid herpesvirus 3 encoded viral interleukin10

Ouyang, Ping, Yang, Ruixue, Yin, Lizi, Geng, Yi, Lai, Weiming, Huang, Xiaoli, Chen, Defang, Fang, Jing, Chen, Zhengli, Tang, Li, He, Min, Huang, Chao, Liu, Wentao, Wang, Kaiyu
Fish & shellfish immunology 2019 v.89 pp. 149-157
Cyprinid herpesvirus 3, Cyprinus carpio, amino acid sequences, bioactive properties, bioinformatics, genes, immune evasion, interleukin-10, koi, quantitative polymerase chain reaction, viruses
Cyprinid herpesvirus 3 (CyHV-3), a virus that encodes an interleukin10 (IL-10) homologue, causes severe economic losses to the common carp and koi culture industry. The present study was devoted to this IL-10 homologue. Recombinant viral IL-10 (vIL-10) protein encoded by CyHV-3 ORF134 gene using prokaryotic expression system was obtained successfully. Bioinformatics analysis revealed that the amino acid sequence of CyHV-3 vIL-10 has low homology with other host IL-10 or viruses encoded IL-10s. However, their tertiary structure is quite similar, suggesting conservative biological functions between IL-10s and vIL-10s. The biological activity of CyHV-3 vIL-10 was detected by using CCK-8 kit and real time quantitative PCR. The results showed that CyHV-3 vIL-10 down regulate epithelioma papulosum cyprini (EPC) cellular activity at 72 h. Moreover, CyHV-3 vIL-10 inhibits the LPS-induced expression of proinflammatory genes, similar to common carp IL-10. Altogether, the results of this study demonstrate that a clear biological activity of CyHV-3 vIL-10 on its host cells and indicates CyHV-3 vIL-10 may play an important role in viral immune evasion.