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High production of optically pure (3R)-acetoin by a newly isolated marine strain of Bacillus subtilis CGMCC 13141

Dai, Jianying, Wang, Zhuangfei, Xiu, Zhi-Long
Bioprocess and biosystems engineering 2019 v.42 no.3 pp. 475-483
Bacillus subtilis, acetoin, aeration, batch fermentation, carbon, catalytic activity, glucose, isomers, nutrients, oxygen
Acetoin is one of the bio-based platform chemicals and its optically pure isomers are important potential intermediates and precursors in the synthesis of novel optically active materials. (3R)-acetoin could be synthesized via enzymatic catalysis, whole-cell catalysis and fermentation. In this study a marine strain of Bacillus subtilis was isolated to produce optically pure (3R)-acetoin with glucose as carbon source. The effects of nutrients on the formation of (3R)-acetoin and conversion of glucose to (3R)-acetoin were evaluated by Plackett–Burman design, and the fermentation medium was optimized by central composite design. The impact of oxygen supply on the production of (3R)-acetoin was studied at different aeration rates. Under the optimal conditions, 83.7 g/L (3R)-acetoin with an optical purity of 99.4% was achieved by fed-batch fermentation, and the conversion of glucose to (3R)-acetoin was 91.5% of the theoretical value. The results indicate the industrial potential of this strain for (3R)-acetoin production via fermentation.