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Anti-Neuroinflammatory Effects of Vanillin Through the Regulation of Inflammatory Factors and NF-κB Signaling in LPS-Stimulated Microglia

Author:
Kim, Mi Eun, Na, Ju Yong, Park, Yong-Duk, Lee, Jun Sik
Source:
Applied biochemistry and biotechnology 2019 v.187 no.3 pp. 884-893
ISSN:
0273-2289
Subject:
Alzheimer disease, Parkinson disease, antineoplastic activity, central nervous system, gene expression, homeostasis, immune response, inducible nitric oxide synthase, inflammation, interleukin-1beta, interleukin-6, lipopolysaccharides, macrophages, messenger RNA, mitogen-activated protein kinase, necrosis, neoplasms, neuroglia, nitric oxide, phosphorylation, prostaglandin synthase, protein content, therapeutics, transcription factor NF-kappa B, tumor necrosis factor-alpha, vanilla beans, vanillin
Abstract:
Microglia, resident macrophages of the central nervous system (CNS), is responsible for immune responses and homeostasis of the CNS. Microglia plays a complex role in neuroinflammation, which has been implicated in neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. Therefore, therapeutic agents that suppress the microglia-mediated inflammatory response could potentially be used in the prevention or treatment of neurodegenerative diseases. Vanillin, a primary component of vanilla bean extract, has anti-inflammatory, anticancer, and antitumor properties. However, the effects of vanillin on the anti-neuroinflammatory responses of microglial cells are still poorly understood. In this study, we investigated the mechanism by which vanillin induces anti-neuroinflammatory responses in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. We found that vanillin significantly decreased the production of nitric oxide and pro-inflammatory cytokines, including interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Vanillin also reduced the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as the mRNA expression levels of IL-1β, TNF-α, and IL-6. Moreover, vanillin inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB. Collectively, these results suggest that vanillin has anti-neuroinflammatory properties and may act as a natural therapeutic agent for neuroinflammatory diseases.
Agid:
6354500