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Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.

Author:
Uysal, Sengul, Ugurlu, Asli, Zengin, Gokhan, Baloglu, Mehmet Cengiz, Altunoglu, Yasemin Celik, Mollica, Adriano, Custodio, Luisa, Neng, Nuno R., Nogueira, José M.F., Mahomoodally, M. Fawzi
Source:
Food and chemical toxicology 2018 v.111 pp. 525-536
ISSN:
0278-6915
Subject:
Bidens tripartita, alpha-glucosidase, antioxidant activity, antioxidants, bioactive compounds, cell lines, cell viability, chlorogenic acid, computer simulation, cytotoxicity, enzyme inhibition, epicatechin, ethyl acetate, high performance liquid chromatography, hydrogen bonding, methanol, neoplasm cells, neoplasms, plasmids, toxicology, viability assays
Abstract:
Bidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silico methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest α-amylase inhibitory activity, while the best α-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to α-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases.
Agid:
6355043