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Separating and Profiling Phosphatidylcholines and Triglycerides from Single Cellular Lipid Droplet by In-Tip Solvent Microextraction Mass Spectrometry

Author:
Zhao, Yaoyao, Chen, Zhen, Wu, Yue, Tsukui, Takayuki, Ma, Xiaoxiao, Zhang, Xinrong, Chiba, Hitoshi, Hui, Shu-Ping
Source:
Analytical chemistry 2019 v.91 no.7 pp. 4466-4471
ISSN:
1520-6882
Subject:
droplets, mammals, mass spectrometry, microextraction, packaging, phosphatidylcholines, solvents, triacylglycerols
Abstract:
The analysis of lipid droplets (LDs) by mass spectrometry at the single LD level is still an analytical challenge. In this work, we developed a novel technique termed in-tip solvent microextraction mass spectrometry for the separation and profiling of phosphatidylcholines and triglycerides within a single LD. This method has been successfully used to analyze LDs in mammalian cells and to compare the profiles of triglycerides and phosphatidylcholines in LDs induced at different conditions. Our method has the potential to be applied to such fields as fundamental lipid biology to further our understanding on the mechanisms of lipid production, lipid packaging, and their pathophysiological roles.
Agid:
6355692