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Development of a Paper-Based Viscometer for Blood Plasma Using Colorimetric Analysis

Kang, Hyunwoong, Jang, Ilhoon, Song, Simon, Bae, Sang-Cheol
Analytical chemistry 2019 v.91 no.7 pp. 4868-4875
aqueous solutions, blood plasma, bovine serum albumin, colorimetry, dimethyl sulfoxide, glycerol, mobile telephones, normal values, paper, patients, viscometers, viscosity
The viscosity of biofluids can be used to acquire meaningful medical information on the conditions of a patient but has seldom been utilized in clinical practices owing to cumbersome measurement procedures and the need for large sample volumes. We present a colorimetric method to measure the viscosity of blood plasma using a paper-based viscometer developed in this study specifically for clinical diagnosis. The proposed analytical device consists of multilayered papers with fluid-loading, -mixing, and -measuring regions, and it can be fabricated readily in a simple manner using three-layered paper channels and tape. Moreover, the colorimetric analysis enables viscosity estimations by analyzing a single optical image. To validate the device performance, we measured the viscosities of fluids such as glycerin aqueous solutions, bovine-serum-albumin solution, dimethyl sulfoxide, and blood plasma. We found that the measured viscosities were in good agreement with the reference values. Finally, we developed a simple smartphone application for the viscosity measurements that helped enhance the convenience and utility of the paper-based viscometer while maintaining the measurement accuracy.