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Human Antibody Bispecifics through Phage Display Selection

Author:
Luthra, Ansha, Langley, David B., Schofield, Peter, Jackson, Jennifer, Abdelatti, Mahmoud, Rouet, Romain, Nevoltris, Damien, Mazigi, Ohan, Crossett, Ben, Christie, Mary, Christ, Daniel
Source:
Biochemistry 2019 v.58 no.13 pp. 1701-1704
ISSN:
1520-4995
Subject:
antibodies, humans, mutation, peptide libraries
Abstract:
We developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics.
Agid:
6355763