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Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization
- Chiles, Eric, Wang, Yujue, Kalemba, Katarzyna M., Kwon, Hyokjoon, Wondisford, Fredric E., Su, Xiaoyang
- Analytical biochemistry 2019 v.575 pp. 40-43
- derivatization, glucose, glucose 6-phosphate, glycerol, ionization, isotope labeling, liquid chromatography, mass spectrometry, metabolism, metabolites, metabolomics, stable isotopes
- Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. This derivatization protocol can be used to measure the concentrations of glucose production in a plethora of sample types for metabolic analysis and is compatible with the general metabolomics workflow. This novel approach allows us to quantitatively study glucose and glycerol metabolism using stable isotope tracers in vivo.