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Antibiofilm activity of the essential oil of Campomanesia aurea O. Berg against microorganisms causing food borne diseases

Kuhn, Daniel, Ziem, Rafaela, Scheibel, Talita, Buhl, Bárbara, Vettorello, Gabriela, Pacheco, Leandra Andressa, Heidrich, Daiane, Kauffmann, Carla, de Freitas, Elisete M., Ethur, Eduardo M., Hoehne, Lucélia
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.108 pp. 247-252
Campomanesia, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella Enteritidis, Staphylococcus aureus, antimicrobial properties, biofilm, chemical composition, ecosystems, essential oils, foodborne illness, gas chromatography-mass spectrometry, gentian violet, in vitro studies, indigenous species, microorganisms, minimum inhibitory concentration, pathogens, plants (botany), sesquiterpenoids
Campomanesia aurea O. Berg is a native plant of the biome Pampa, which belongs to the family Myrtaceae. The present study aimed to evaluate the antimicrobial and antibiofilme activity of the essential oil of Campomanesia aurea (EOCA) against Listeria monocytogenes ATCC 19114, Staphylococcus aureus ATCC 25923, Salmonella enteritidis ATCC 13076 and Pseudomonas aeruginosa ATCC 27853. An analysis of the chemical composition of the EOCA was realized through gas chromatography coupled to mass spectrometry (GC-MS). The analysis for in vitro evaluation of the antimicrobial and antibiofilm activity was realized by determination of the minimal inhibitory concentration (MIC) and that of the antibiofilm through utilization of 96-well plates with crystal violet, respectively. The action of standard (E)–nerolidol (major compound of the EOCA) was also tested. GC-MS analysis revealed the presence of the sesquiterpene (E)-nerolidol (56.04%) as the main compound in the EOCA. Antimicrobial activity of the EOCA against L. monocytogenes (MIC 5.0 mg mL−1) and S. aureus (MIC 0.7 mg mL−1) was observed. Inhibition of biofilm formation against L. monocytogenes, S. aureus and S. enteritidis could be observed for EOCA and (E)-nerolidol. The results demonstrate that the EOCA was efficient against inhibition of biofilm formation for most of the tested pathogens.