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Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane

Author:
Ng, I-Son, Tang, Malcolm S.Y., Show, Pau Loke, Chiou, Zih-Ming, Tsai, Jung-Chin, Chang, Yu-Kaung
Source:
International journal of biological macromolecules 2019 v.132 pp. 615-628
ISSN:
0141-8130
Subject:
Arthrospira platensis, algae, chitosan, chromatography, mass transfer, nanofibers, pH, permeability, proteins, sodium chloride
Abstract:
In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10−2–2.04 × 10−1 mL/min·cm2) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.
Agid:
6358286