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Comparison of plasma and urinary microRNA-483-5p for the diagnosis of adrenocortical malignancy
- Decmann, Abel, Bancos, Irina, Khanna, Aakanksha, Thomas, Melinda A., Turai, Péter, Perge, Pál, Pintér, József Zsolt, Tóth, Miklós, Patócs, Attila, Igaz, Peter
- Journal of biotechnology 2019 v.297 pp. 49-53
- adenoma, biomarkers, carcinoma, microRNA, patients, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction
- Minimally invasive circulating microRNAs might be used for the preoperative differentiation of adrenocortical carcinoma (ACC) and adrenocortical adenoma (ACA). So far, the best blood-borne microRNA biomarker of ACC is circulating hsa-miR-483-5p. The expression of urinary hsa-miR-483-5p as a non-invasive marker of malignancy and its correlation with plasma hsa-miR-483-5p, has not been investigated, yet.Our aim was to investigate the expression of urinary hsa-miR-483-5p and its correlation with its plasma counterpart.Plasma and urinary samples from 23 ACC and 23 ACA patients were analysed using real-time RT-qPCR. To evaluate the diagnostic applicability of hsa-miR-483-5p, ROC-analysis was performed.Significant overexpression of hsa-miR-483-5p was observed in carcinoma patients’ plasma samples compared to adenoma patients’ (p < 0.0001, sensitivity: 87%, specificity: 78.3%). In urinary samples, however, no significant difference could be detected between ACC and ACA patients.Plasma hsa-miR-483-5p has been confirmed as significantly overexpressed in adrenocortical cancer patients and thus might be exploited as a minimally invasive preoperative marker of malignancy. The applicability of urinary hsa-miR-483-5p for the diagnosis of adrenocortical malignancy could not be confirmed.