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The complete sequences of two divergent variants of the rhabdovirus raspberry vein chlorosis virus and the design of improved primers for virus detection
- Jones, Sue, McGavin, Wendy, MacFarlane, Stuart
- Virus research 2019 v.265 pp. 162-165
- Strawberry crinkle virus, alfalfa, chlorosis, nucleotide sequences, oligodeoxyribonucleotides, raspberries, reverse transcriptase polymerase chain reaction, sequence analysis, viruses
- The complete sequence was obtained for two variants of raspberry vein chlorosis virus (RVCV), confirming that this virus is a rhabdovirus most closely related to the cytorhabdoviruses alfalfa dwarf virus and strawberry crinkle virus. The two RVCV variants share only a 68% nucleotide sequence identity so that the previously published RT-PCR diagnostic test for this virus was not able to efficiently detect both variants. Using the new, complete sequence information several new primer sets have been designed that allow a much improved RVCV detection.