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Intermittent fasting beneficial effects on the steatosis and inflammation of the liver in mice fed a high-fat or a high-fructose diet

Marinho, Thatiany de Souza, Ornellas, Fernanda, Barbosa-Da-Silva, Sandra, Mandarim-De-Lacerda, Carlos A., Aguila, Marcia Barbosa
Nutrition 2019
adiponectin, beta oxidation, cholesterol, fasting, fatty liver, food intake, fructose, glucose, glucose tolerance tests, high fructose diet, inflammation, insulin, leptin, lipid composition, lipogenesis, liver, males, mice, nutritional intervention, obesogenic diet, triacylglycerols
Intermittent fasting (‘IF’) is a nutritional intervention whose significant metabolic effects on the liver are not yet fully understood.We used cellular and molecular techniques to investigate the effects of ‘IF’ on body mass (BM), lipid profile, glucose metabolism, and the liver lipogenesis, beta-oxidation, and inflammation. Male C57BL/6 mice three-month-old were fed Control (C, 10% KCal fat), High-fat (HF, 50% KCal fat) or High-fructose (HFr, 50% KCal fructose) diets for eight weeks, then half of the animals were submitted to ‘IF’ (one day fed, one day fast) for an additional four weeks.Although food intake in the fed day has not been different between the groups, HF and HFr showed diminished BM, total cholesterol and triacylglycerol. Also, plasmatic adiponectin increased in HFr and leptin decreased in HF. Oral glucose tolerance test and insulin were ameliorated by ‘IF,’ regardless of the diet consumed (HF or HFr), as well as decreased hepatic lipogenesis and increased beta-oxidation markers, resulting in a reduction of the hepatic steatosis and inflammation.There were beneficial effects of 'IF' even with the continuity of the obesogenic diet and proinflammatory diet in mice. The beneficial effects of 'IF' on glucose metabolism and the liver metabolism and inflammation recommend 'IF' as coadjutant factor treating hepatic metabolic troubles and steatosis.