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An environmentally friendly deproteinization and decolorization method for polysaccharides of Typha angustifolia based on a metal ion-chelating resin adsorption

Shi, Yuqi, Yuan, Zhixiang, Xu, Tingting, Qu, Runbo, Yuan, Jun, Cai, Fuhong, Wang, Yunpeng, Wang, Xiaoli
Industrial crops and products 2019 v.134 pp. 160-167
Typha angustifolia, adsorption, decolorization, moieties, molecular weight, pH, polysaccharides, sustainable technology, temperature, toxicity
This study developed an environmentally friendly method for the deproteinization and decolorization of polysaccharide (PTA) from Typha angustifolia using a metal ion-chelating resin. Based on single-factor experiments, the optimal adsorption parameters of the developed method were determined as follows: a concentration of crude polysaccharide of 1.5 mg/mL; an adsorption time of 30 min; an initial pH of 7.0; an adsorption temperature of 30 °C; a flow rate of 1.5 BV/h; a diameter-to-height ratio of the resin column of 1:15. Under these optimal conditions, the proposed method exhibited a higher deproteinization ratio, decolorization ratio, recovery ratio and comprehensive adsorption effect index compared to the commonly used Sevag method. No significant differences in the monosaccharide composition, characteristic functional groups and molecular weight of PTA were observed between the proposed method and the Sevag method. Moreover, the proposed chelating resin method does not consume any toxic organic reagents. The results suggest that this method is promising for the deproteinization and decolorization of PTA.