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Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production
- Wielanek, Marzena, Królicka, Aleksandra, Bergier, Katarzyna, Gajewska, Ewa, Skłodowska, Maria
- Biotechnology letters 2009 v.31 no.6 pp. 917-921
- Arabis alpina subsp. caucasica, Barbarea, Nasturtium officinale, Rhizobium rhizogenes, biosynthesis, cysteine, glucosinolates, methionine, phenylalanine, roots
- Hairy roots of Nasturtium officinale, Barbarea verna and Arabis caucasica with active glucosinolate-myrosinase system were obtained after transformation with Agrobacterium rhizogenes. Hairy roots of N. officinale produced phenylalanine-derived gluconasturtiin and glucotropaeolin (max. 24 and 7 mg g⁻¹ DW). B. verna and A. caucasica hairy roots produced gluconasturtiin (max. 41 mg g⁻¹ DW) and methionine-derived glucoiberverin (max. 32 mg g⁻¹ DW), respectively. Treatment of the roots with amino acid precursors of glucosinolate or/and cysteine biosynthesis increased levels of glucosinolate production, combinations of phenylalanine with cysteine (for gluconasturtiin and glucotropaeolin) and methionine with o-acetylserine (for glucoiberverin) were the most effective.