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Antibiotic supplement in feed can perturb the intestinal microbial composition and function in Pacific white shrimp

Author:
Zeng, Shenzheng, Hou, Dongwei, Liu, Jian, Ji, Peilin, Weng, Shaoping, He, Jianguo, Huang, Zhijian
Source:
Applied microbiology and biotechnology 2019 v.103 no.7 pp. 3111-3122
ISSN:
0175-7598
Subject:
Litopenaeus vannamei, amino acid metabolism, antibiotic resistance genes, antibiotics, aquaculture, carbohydrate metabolism, ciprofloxacin, feed supplements, intestinal microorganisms, intestines, microbial communities, ribosomal RNA, shrimp
Abstract:
The intestinal microbiota plays crucial roles in host health. The Pacific white shrimp is one of the most profitable aquaculture species in the world. Antibiotic supplement in feed is an optional practice to treat shrimp bacterial diseases. However, little is known about antibiotic effects on intestinal microbiota in pacific white shrimp. Here, shrimps were given feed supplemented with ciprofloxacin (Cip) (40 and 80 mg kg⁻¹) and sulfonamide (Sul) (200 and 400 mg kg⁻¹) to investigate the microbial community by targeting the V4 region of 16S rRNA genes. Within 4 days after feeding with normal feed and with antibiotics, antibiotic concentrations of Cip and Sul groups in the intestine dropped sharply. Significantly, increased abundance of antibiotic resistance genes (ARGs) of ciprofloxacin (qnrB, qnrD, and qnrS) and sulfonamide (sul1, sul2, and sul3) was observed in Cip and Sul groups (P < 0.05). A total of 3191 operational taxonomic units (OTUs) were obtained and 41 phyla were identified from 63 samples in shrimp intestine. The numbers of OTUs and Shannon index decreased rapidly at day 1 (the first day after feeding with antibiotics) and increased at day 3 (the third day after feeding with antibiotics). The relative abundance of dominant phyla and genera in Cip and Sul groups were significantly different from that in the control group (Ctrl). Furthermore, functional potentials that were related to amino acid metabolism, carbohydrate metabolism, and cellular processes and signaling varied significantly in Cip and Sul groups. These results point to an antibiotic-induced shift in shrimp intestinal microbiota, which highlights the importance of considering the microbiota in shrimp health management.
Agid:
6360258