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Inheritance of Provisia™ rice resistance to quizalofop-p-ethyl under laboratory and greenhouse environments

Camacho, Jose R., Linscombe, Steve D., Sanabria, Yamid, Mosquera, Paola A., Oard, James H.
Euphytica 2019 v.215 no.4 pp. 83
Oryza sativa, breeding lines, dominant genes, enzyme inhibitors, genetic markers, grass weeds, greenhouse experimentation, herbicide resistance, hybrids, maternal effect, parents, plant breeding, quizalofop, rice, seedlings, seeds, single nucleotide polymorphism, transgenic plants, weed control, United States
The BASF Corporation recently developed Provisia™ rice that is resistant to the ACCase inhibitor quizalofop-p-ethyl (QPE) for control of grassy weeds and that is complementary to the existing Clearfield® technology. The objective of this research was to determine the inheritance of resistance to QPE in different populations of rice evaluated under laboratory and greenhouse conditions. Five QPE-resistant lines and 13 susceptible U.S. varieties and breeding lines were used to develop 16 F₁ populations that showed dominant gene control for QPE resistance in the greenhouse. Germinating seeds and young seedlings from 16 F₂ and 12 BC₁F₁ populations, along with corresponding susceptible and resistant parents, were exposed to minimum lethal concentrations of QPE. The results indicated that resistance to QPE under the two environments was governed by a single dominant Mendelian gene with no observable maternal effects. Allele-specific SNP markers for QPE resistance were developed that also followed Mendelian segregation in four F₂ and six BC₁F₁ populations. Results from this study have direct implications for breeding strategies of inbred and hybrids varieties resistant to QPE in the laboratory and greenhouse and for effective stewardship of the Provisia™ technology.