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Label-free detection of exosomes using a surface plasmon resonance biosensor

Author:
Sina, Abu Ali Ibn, Vaidyanathan, Ramanathan, Wuethrich, Alain, Carrascosa, Laura G., Trau, Matt
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.7 pp. 1311-1318
ISSN:
1618-2642
Subject:
biosensors, blood serum, breast neoplasms, exosomes, messenger RNA, metastasis, microRNA, proteins, surface plasmon resonance
Abstract:
The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs, and proteins) to remote cells for secondary metastasis. Herein, we report a simple method for the real-time and label-free detection of clinically relevant exosomes using a surface plasmon resonance (SPR) biosensor. Our method shows high specificity in detecting BT474 breast cancer cell–derived exosomes particularly from complex biological samples (e.g. exosome spiked in serum). This approach exhibits high sensitivity by detecting as low as 8280 exosomes/μL which may potentially be suitable for clinical analysis. We believe that this label-free and real-time method along with the high specificity and sensitivity may potentially be useful for clinical settings.
Agid:
6362698