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Taxonomic characterization and antimicrobial compound production from Streptomyces chumphonensis BDK01 isolated from marine sediment

Author:
Manikandan, Madheslu, Gowdaman, Vasudevan, Duraimurugan, Kasiviswanathan, Prabagaran, Solai Ramatchandirane
Source:
3 Biotech 2019 v.9 no.5 pp. 167
ISSN:
2190-572X
Subject:
Bacillus pumilus, Enterobacter cloacae, Escherichia coli, Proteus vulgaris, Shigella flexneri, Streptomyces, alcohols, antimicrobial properties, bioactive compounds, calcium carbonate, casein, cell lines, cytotoxicity, ethyl acetate, inhibitory concentration 50, inoculum, marine sediments, metabolites, methicillin-resistant Staphylococcus aureus, models, pH, phenotype, sodium chloride, spectral analysis, starch, temperature, Bay of Bengal
Abstract:
Streptomyces sp. isolated from marine sediment collected from Palk Strait, Bay of Bengal was investigated for its antagonistic potential. The isolate exhibited antimicrobial activity against selected bacterial strains of clinical importance such as Staphylococcus aureus MTCC 3160, Bacillus pumilus NCIM 2327, S. aureus (methicillin resistant), Escherichia coli MTCC 1698, E. coli (ESBL), Shigella flexneri MTCC 1457, Proteus vulgaris and Enterobacter cloacae. Phenotypic and molecular characterization ascertained the isolate BDK01 as Streptomyces chumphonensis. Media optimization with one variable-at-a-time strategy was attempted to identify the ideal concentrations of starch (5–15 g/l), casein (0.01–0.05 g/l), NaCl 1.0–3.0 g/l, pH (4.0–9.0 g/l), temperature (25–45 °C) and inoculum level (0.5–5 ml) towards achieving maximum antimicrobial compound production. Statistical optimization of production media was carried by establishing an 11 variables 17 run experiment through PB model which evinced starch, calcium carbonate, pH and inoculum concentration that highly influenced bioactive compound production. Spectral data of active ethyl acetate extract revealed the presence of various bioactive compounds such as Salicyl alcohol, N-phenyl benzamide. 6-Octadecenoic acid, (Z), 1,3,5-Cycloheptatriene. Antiproliferation activity of active fraction against MCF-7 Cell line exhibited IC₅₀ value of 9.5 µg/ml. Overall, it is observed that the marine actinomycete S. chumphonensis BDK01 could be employable as promising strain for novel antimicrobial and cytotoxic metabolites.
Agid:
6362883