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Effect of nitrogen concentration, two‐stage and prolonged cultivation on growth rate, lipid and protein content of Chlorella vulgaris

Author:
Metsoviti, Maria N, Katsoulas, Nikolaos, Karapanagiotidis, Ioannis T, Papapolymerou, Georgios
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.5 pp. 1466-1473
ISSN:
0268-2575
Subject:
Chlorella vulgaris, biomass production, culture media, developmental stages, kinetics, lipid content, lipids, nitrogen content, protein content, specific growth rate
Abstract:
BACKGROUND: The effects of the initial nitrogen concentration, as well as of two‐stage and prolonged cultivation on the growth rate, lipid and protein content of Chlorella vulgaris were examined. Additionally, a kinetic model of specific growth rate in the exponential growth phase (µₑₓₚ) was developed as a function of initial nitrogen concentration. RESULTS: As the initial nitrogen concentration in the culture medium increased, the total biomass production and µₑₓₚ went through a maximum at 300 mg N L⁻¹ and at 200 mg N L⁻¹, respectively. The lipid content was found to be highly dependent on the initial nitrogen concentration and increased from 9.0% to 29.6% as the nitrogen concentration varied from 800 to 10 mg N L⁻¹, respectively. Cultivation in two stages resulted in an increase of the lipid content of the biomass from 15.4% to 24.9%, while prolonged cultivation led to an increase of the lipid content from 15.4% to 23.0%. CONCLUSION: Cultivation under optimum nitrogen concentration can lead to increased biomass production. In addition, optimization of lipid production can be carried out by a two‐stage cultivation mode or by prolonged cultivation. © 2018 Society of Chemical Industry
Agid:
6364309